Molecular Cloning and Expression of the Leishmania infantum KMP-11 Gene
نویسندگان
چکیده
منابع مشابه
molecular cloning and expression of the leishmania infantum kmp-11 gene
conclusions the results of the present study will increase our knowledge about molecular cloning and expression of the l. infantum kmp-11 gene, and this may be used as an effective target for controlling visceral leishmaniasis. results the kmp-11 gene was successfully subcloned in pcdna3 as a eukaryotic expression vector. recombinant kmp-11 protein was confirmed by the reverse transcriptase pol...
متن کاملMolecular Cloning and Expression of the Leishmania infantum KMP-11 Gene
Background: Visceral leishmaniasis (Kala-azar) is one of the most serious tropical diseases, and it can lead to death. The kinetoplastid membrane protein-11 (KMP-11) is highly conserved in all stages of the Leishmania life cycle. Objectives: In the present study, the KMP-11 gene was extracted from Leishmania infantum and then, cloned and expressed in an expression vector.The main objective of t...
متن کاملcloning and expression of leishmania infantum lpg3 gene by the lizard leishmania expression system
background: various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. in the present study, we used a new protein expression system based on the iranian lizard leishmania, a trypanosomatid protozoan as a host, for the expression of lpg3 gene from leishmania infantum (l.infantum). methods: the lpg3 gene was cloned in the expression cass...
متن کاملCloning and Expression of Leishmania infantum LPG3 Gene by the Lizard Leishmania Expression System
BACKGROUND Various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. In the present study, we used a new protein expression system based on the Iranian Lizard Leishmania, a trypanosomatid protozoan as a host, for the expression of LPG3 gene from Leishmania infantum (L.infantum). METHODS The LPG3 gene was cloned in the expression cass...
متن کاملMolecular Cloning and Characterization of P4 Nuclease from Leishmania infantum
Parasite of the genus Leishmania is reliant on the salvage pathway for recycling of ribonucleotides. A class I nuclease enzyme also known as P4 nuclease is involved in salvage of purines in cutaneous Leishmania species but the relevant enzymes have not been characterized in Leishmania infantum (L. infantum). The aim of this study was to clone and characterize the gene encoding class I nuclease ...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2013
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.4798